We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Role of ribosome recycling factor (RRF) in translational coupling.
- Authors
Inokuchi, Yoshio; Hirashima, Akikazu; Sekine, Yasuhiko; Janosi, Laszlo; Kaji, Akira
- Abstract
RNA phage GA coat and lysis protein expression are translationally coupled through an overlapping termination and initiation codon UAAUG. Essential for this coupling are the proximity of the termination codon of the upstream coat gene to the initiation codon of the lysis gene (either a <3 nucleotide separation or physical closeness through a possible hairpin structure) but not the ShineDalgarno sequence. This suggests that the ribosomes completing the coat gene translation are exclusively responsible for translation of the lysis gene. Inactivation of ribosome recycling factor (RRF), which normally releases ribosomes at the termination codon, did not influence the expression of the reporter gene fused to the lysis gene. This suggests the possibility that RRF may not release ribosomes from the junction UAAUG. However, RRF is essential for correct ribosomal recognition of the AUG codon as the initiation site for the lysis gene.
- Subjects
RNA; PROTEINS; GENES; RIBOSOMES; PSEUDOMONAS aeruginosa
- Publication
EMBO Journal, 2000, Vol 19, Issue 14, p3788
- ISSN
0261-4189
- Publication type
Article
- DOI
10.1093/emboj/19.14.3788