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- Title
Endocrine Disruption of Water and Sediment Extracts in a Non-Radioactive Dot Blot/RNAse Protection-Assay Using Isolated Hepatocytes of Rainbow Trout: Deficiencies between bioanalytical effectiveness and chemically determined concentrations and how to explain them.
- Authors
Hollert, Henner; Dürr, Matthias; Holtey-Weber, Roman; Islinger, Markus; Brack, Werner; Färber, Harald; Erdinger, Lothar; Braunbeck, Thomas
- Abstract
The article investigates the ecological hazard potential of endocrine-disrupting compounds present in sediment and water in a non-radioactive dot blot/RNAse protection-assay using isolated hepatocytes. Near-surface sediment samples of the small streams were collected by means of a Van Veen grab sampler. A total of 10 water samples were collected at each location within the catchment area of river Neckar, Southern Germany, over a period of 3 hours and combined to a mixed sample. The result indicates, in small creek systems, that wastewater discharges may result in a significant increase of the endocrine load of the whole water.
- Subjects
GERMANY; ENDOCRINE toxicology; RIVERS; LIVER cells; FIRE assay; RNA; CONSTRUCTED wetlands; HEALTH service areas
- Publication
Environmental Science & Pollution Research, 2005, Vol 12, Issue 6, p347
- ISSN
0944-1344
- Publication type
Article
- DOI
10.1065/espr2005.07.273