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- Title
Trans-SILAC: sorting out the non-cell-autonomous proteome.
- Authors
Rechavi, Oded; Kalman, Matan; Yuan Fang; Vernitsky, Helly; Jacob-Hirsch, Jasmine; Foster, Leonard J.; Kloog, Yoel; Goldstein, Itamar
- Abstract
Non-cell-autonomous proteins are incorporated into cells that form tight contacts or are invaded by bacteria, but identifying the full repertoire of transferred proteins has been a challenge. Here we introduce a quantitative proteomics approach to sort out non-cell-autonomous proteins synthesized by other cells or intracellular pathogens. Our approach combines stable-isotope labeling of amino acids in cell culture (SILAC), high-purity cell sorting and bioinformatics analysis to identify the repertoire of relevant non-cell-autonomous proteins. This 'trans-SILAC' method allowed us to discover many proteins transferred from human B to natural killer cells and to measure biosynthesis rates of Salmonella enterica proteins in infected human cells. Trans-SILAC should be a useful method to examine protein exchange between different cells of multicellular organisms or pathogen and host.
- Subjects
PROTEINS; PROTEOMICS; INTRACELLULAR pathogens; BIOINFORMATICS; KILLER cells
- Publication
Nature Methods, 2010, Vol 7, Issue 11, p923
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1513