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- Title
Tomatidine inhibits tumor necrosis factor-α-induced apoptosis in C<sub>2</sub>C<sub>12</sub> myoblasts via ameliorating endoplasmic reticulum stress.
- Authors
Song, Seung-Eun; Shin, Su-Kyung; Cho, Hyun-Woo; Im, Seung-Soon; Bae, Jae-Hoon; Woo, Seon Min; Kwon, Taeg-Kyu; Song, Dae-Kyu
- Abstract
In this study, we examined the effect of tomatidine on tumor necrosis factor (TNF)-α-induced apoptosis in C2C12 myoblasts. TNF-α treatment increased cleaved caspase 3 and cleaved poly (ADP-ribose) polymerase (PARP) protein levels in a dose- and time-dependent manner. Pretreatment of cells with 10 μM tomatidine prevented TNF-α-induced apoptosis, caspase 3 cleavage, and PARP cleavage. Cells were treated with 100 ng/mL TNF-α for 24 h, and flow cytometry was utilized to assess apoptosis using annexin-V and 7-aminoactinomycin D. TNF-α up-regulated activating transcription factor 4 (ATF4) and C/EBP homologous protein (CHOP) expression. This effect was suppressed by pretreatment with tomatidine. Pretreatment with 4-phenylbutyric acid (a chemical chaperone) also inhibited TNF-α-induced cleavage of caspase 3 and PARP and up-regulation of ATF4 and CHOP expression. In addition, tomatidine-mediated inhibition of phosphorylation of c-Jun amino terminal kinase (JNK) attenuated TNF-α-induced cleavage of PARP and caspase 3. However, tomatidine did not affect NF-κB activation in TNF-α-treated C2C12 myoblast cells. Taken together, the present study demonstrates that tomatidine attenuates TNF-α-induced apoptosis through down-regulation of CHOP expression and inhibition of JNK activation.
- Subjects
TUMOR necrosis factors; APOPTOSIS; MYOBLASTS; ENDOPLASMIC reticulum; CCAAT enhancer binding proteins
- Publication
Molecular & Cellular Biochemistry, 2018, Vol 444, Issue 1/2, p17
- ISSN
0300-8177
- Publication type
Article
- DOI
10.1007/s11010-017-3226-3