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- Title
Enzymatic synthesis of polypeptides in natural deep eutectic solvents.
- Authors
Hernández-Alonso, Ángel; Pérez-García, Vanessa; López-Medina, Jared; Cedeño-Caero, Luis; Trapala, Jonathan; Montiel, Carmina; Bustos-Jaimes, Ismael; Gimeno, Miquel
- Abstract
We are first to report the protease-catalyzed polypeptide synthesis in the natural deep eutectic solvent (DES) choline chloride/glycerol (ChCl:Gly) at a 1:2 molar ratio with 30 vol% of water using subtilisin Carlsberg (alkaline protease from Bacillus licheniformis). Poly-L-phenylalanine, poly-a-L-lysine, and the co-polypeptides poly(L-phenylalanine-a-L-lysine) as model polypeptides are produced from amino acid ester substrates in 40–70% yield and molecular weights ranging from 5 to 14 KDa according to dynamic light scattering (DLS). The natural DES is composed of 30 vol% water, whereas no polypeptides are formed at lower water contents. Poly-L-phenylalanine displays supramolecular self-assembly into homogenous nanotubes in water/isopropyl alcohol mixtures. This enzymatic route to polypeptides has advantages over previous reports in water and polar compressed fluids because of the higher molecular weights and greener process, respectively.
- Subjects
EUTECTICS; POLYPEPTIDES; SUBCOOLED liquids; CHOLINE chloride; ALKALINE protease; BACILLUS licheniformis
- Publication
Journal of Polymer Research, 2023, Vol 30, Issue 11, p1
- ISSN
1022-9760
- Publication type
Article
- DOI
10.1007/s10965-023-03808-1