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- Title
Somatic embryogenesis from immature and mature zygotic embryos of Cryptomeria japonica I: Embryogenic cell induction and its morphological characteristics.
- Authors
Ogita, Shinjiro; Ishikawa, Hisako; Kubo, Takafumi; Sasamoto, Hamako
- Abstract
Embryogenic cells (ECs) of sugi ( Cryptomeria japonica D. Don) were induced from immature and mature zygotic embryos cultured on different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D)-containing modified Campbell and Durzan medium. The rate of induction of ECs varied depending on the stage of embryos collected. The highest percentage of induction (35%) was obtained with immature zygotic embryos collected July 18 and July 30, 1997, when 1 μM of 2,4-D was added to the induction medium. The ECs easily proliferated when subcultured in a medium of the same composition as the induction medium within 3 weeks. Morphological characteristics of nonembryogenic cells and embryogenic cells of different developmental stages were studied under an inverted fluorescence microscope.
- Publication
Journal of Wood Science, 1999, Vol 45, Issue 2, p87
- ISSN
1435-0211
- Publication type
Article
- DOI
10.1007/BF01192323