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- Title
Y-box-binding protein 1 stimulates abasic site cleavage.
- Authors
Alemasova, E.; Naumenko, K.; Moor, N.; Lavrik, O.
- Abstract
Apurinic/apyrimidinic (AP) sites are among the most frequent DNA lesions. The first step in the AP site repair involves the magnesium-dependent enzyme AP endonuclease 1 (APE1) that catalyzes hydrolytic cleavage of the DNA phosphodiester bond at the 5′ side of the AP site, thereby generating a single-strand DNA break flanked by the 3′-OH and 5′-deoxyribose phosphate (dRP) groups. Increased APE1 activity in cancer cells might correlate with tumor chemoresistance to DNA-damaging treatment. It has been previously shown that the multifunctional oncoprotein Y-box-binding protein 1 (YB-1) interacts with APE1 and inhibits APE1-catalyzed hydrolysis of AP sites in single-stranded DNAs. In this work, we demonstrated that YB-1 stabilizes the APE1 complex with double-stranded DNAs containing the AP sites and stimulates cleavage of these AP sites at low magnesium concentrations.
- Subjects
APURINIC acid; BINDING sites; DNA damage; ENDONUCLEASES; PHOSPHODIESTERS
- Publication
Biochemistry (00062979), 2017, Vol 82, Issue 12, p1521
- ISSN
0006-2979
- Publication type
Article
- DOI
10.1134/S0006297917120112