We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
High-level recombinant production of squalene using selected <italic>Saccharomyces cerevisiae</italic> strains.
- Authors
Han, Jong Yun; Seo, Sung Hwa; Song, Jae Myeong; Lee, Hongweon; Choi, Eui-Sung
- Abstract
For recombinant production of squalene, which is a triterpenoid compound with increasing industrial applications, in microorganisms generally recognized as safe, we screened <italic>Saccharomyces cerevisiae</italic> strains to determine their suitability. A strong strain dependence was observed in squalene productivity among <italic>Saccharomyces cerevisiae</italic> strains upon overexpression of genes important for isoprenoid biosynthesis. In particular, a high level of squalene production (400 ± 45 mg/L) was obtained in shake flasks with the Y2805 strain overexpressing genes encoding a bacterial farnesyl diphosphate synthase (<italic>ispA</italic>) and a truncated form of hydroxyl-3-methylglutaryl-CoA reductase (<italic>tHMG1</italic>). Partial inhibition of squalene epoxidase by terbinafine further increased squalene production by up to 1.9-fold (756 ± 36 mg/L). Furthermore, squalene production of 2011 ± 75 or 1026 ± 37 mg/L was obtained from 5-L fed-batch fermentations in the presence or absence of terbinafine supplementation, respectively. These results suggest that the Y2805 strain has potential as a new alternative source of squalene production.
- Subjects
SACCHAROMYCES cerevisiae; SQUALENE; GENETIC overexpression; TERBINAFINE; ISOPENTENOIDS
- Publication
Journal of Industrial Microbiology & Biotechnology, 2018, Vol 45, Issue 4, p239
- ISSN
1367-5435
- Publication type
Article
- DOI
10.1007/s10295-018-2018-4