We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Perturbation of cytosolic calcium by 2-aminoethoxydiphenyl borate and caffeine affects zebrafish myofibril alignment.
- Authors
Wu, Hsin‐Ju; Fong, Tsorng‐Harn; Chen, Shen‐Liang; Wei, Jen‐Cheng; Wang, I‐Jong; Wen, Chi‐Chung; Chang, Chao‐Yuan; Chen, Xing‐Guang; Chen, Wei‐Yu; Chen, Hui‐Min; Horng, Juin‐Lin; Wang, Yun‐Hsin; Chen, Yau‐Hung
- Abstract
The objective of the current study was to investigate the effects of Ca2+ levels on myofibril alignment during zebrafish embryogenesis. To investigate how altered cytoplasmic Ca2+ levels affect myofibril alignment, we exposed zebrafish embryos to 2-aminothoxyldiphenyl borate (2-APB; an inositol 1,4,5-trisphosphate receptor inhibitor that reduces cytosolic Ca2+ levels) and caffeine (a ryanodine receptor activator that enhances cytosolic Ca2+ levels). The results demonstrated that the most evident changes in zebrafish embryos treated with 2-APB were shorter body length, curved trunk and malformed somite boundary. In contrast, such malformed phenotypes were evident neither in untreated controls nor in caffeine-treated embryos. Subtle morphological changes, including changes in muscle fibers, F-actin and ultrastructures were easily observed by staining with specific monoclonal antibodies (F59 and α-laminin), fluorescent probes (phalloidin) and by transmission electron microscopy. Our data suggested that: (1) the exposure to 2-APB and/or caffeine led to myofibril misalignment; (2) 2-APB-treated embryos displayed split and short myofibril phenotypes, whereas muscle fibers from caffeine-treated embryos were twisted and wavy; and (3) zebrafish embryos co-exposed to 2-APB and caffeine resulted in normal myofibril alignment. In conclusion, we proposed that cytosolic Ca2+ is important for myogenesis, particularly for myofibril alignment. Copyright © 2014 John Wiley & Sons, Ltd.
- Subjects
BORATES; CAFFEINE; ZEBRA danio; PHENOTYPES; TRANSMISSION electron microscopy
- Publication
Journal of Applied Toxicology, 2015, Vol 35, Issue 3, p287
- ISSN
0260-437X
- Publication type
Article
- DOI
10.1002/jat.3057