We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Plant-Produced Viral Nanoparticles as a Functionalized Catalytic Support for Metabolic Engineering.
- Authors
Sator, Christian; Lico, Chiara; Pannucci, Elisa; Marchetti, Luca; Baschieri, Selene; Warzecha, Heribert; Santi, Luca
- Abstract
Substrate channeling could be very useful for plant metabolic engineering; hence, we propose that functionalized supramolecular self-assembly scaffolds can act as enzymatic hubs able to perform reactions in close contiguity. Virus nanoparticles (VNPs) offer an opportunity in this context, and we present a functionalization strategy to display different enzymes on the outer surface of three different VNPs produced in plants. Tomato bushy stunt virus (TBSV) and Potato virus X (PVX) plant viruses were functionalized by the genetic fusion of the E-coil peptide coding sequence to their respective coat proteins genes, while the enzyme lichenase was tagged with the K-coil peptide. Immobilized E-coil VNPs were able to interact in vitro with the plant-produced functionalized lichenase, and catalysis was demonstrated by employing a lichenase assay. To prove this concept in planta, the Hepatitis B core (HBc) virus-like particles (VLPs) were similarly functionalized by genetic fusion with the E-coil sequence, while acyl-activating enzyme 1, olivetolic acid synthase, and olivetolic acid cyclase enzymes were tagged with the K-coil. The transient co-expression of the K-coil-enzymes together with E-coil-VLPs allowed the establishment of the heterologous cannabinoid precursor biosynthetic pathway. Noteworthy, a significantly higher yield of olivetolic acid glucoside was achieved when the scaffold E-coil-VLPs were employed.
- Subjects
POTATO virus X; PLANT viruses; VIRUS-like particles; CANNABINOID receptors; AMINO acid sequence; PEPTIDES; COAT proteins (Viruses)
- Publication
Plants (2223-7747), 2024, Vol 13, Issue 4, p503
- ISSN
2223-7747
- Publication type
Article
- DOI
10.3390/plants13040503