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- Title
Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality.
- Authors
Cruz, Sara Michelly; Nery, Marcela Carlota; de Souza Rocha, Adriana; de Resende Von Pinho, Édila Vilela; de Resende Andrade, Paulo César; dos Santos Dias, Denise Cunha Fernandes
- Abstract
The aim of this study was adapting the methodology of the accelerated aging test (AA) and electrical conductivity test (EC) to evaluate seed quality of crambe, as well as verifying enzymatic activity in relation to vigor differences. Crambe seed lots, cv. FMS Brilhante, were evaluated by the AA test, for periods of 0, 24, 48, 72 and 96 h, at 42 °C, with and without NaCl saturated solution; and the EC test, by soaking seeds into deionized water (mL) in the ratios of 25/25, 25/50, 50/50 and 50/75 (seed/water) for 2, 4, 6, 8, 10, 12, 14, 16 and 18 h. The electrophoretic profile for isozymes esterase, superoxide dismutase, catalase, isocitrate lyase, alcohol dehydrogenase and malate dehydrogenase was evaluated on polyacrylamide gel (7.5%). The AA test conducted at 42 °C, for 96 h, was efficient to detect differences on seed quality levels; however, the EC test was not suitable to evaluate vigor of those seeds. The bands stained for isozymes esterase, superoxide dismutase and catalase were evident for the more vigorous seeds; and the bands for isozymes isocitrate lyase and alcohol dehydrogenase were less evident for the less vigorous seeds. For enzyme malate dehydrogenase enzyme intensity of bands was similar.
- Subjects
CRAMBE; SEED quality; AGING in plants; ELECTRIC conductivity research; PLANT isozymes; POLYACRYLAMIDE; MALATE dehydrogenase
- Publication
Journal of Seed Science, 2013, Vol 35, Issue 4, p485
- ISSN
2317-1537
- Publication type
Article
- DOI
10.1590/S2317-15372013000400010