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- Title
A low-cost, open-source evolutionary bioreactor and its educational use.
- Authors
GOPALAKRISHNAN, VISHHVAAN; CROZIER, DENA; CARD, KYLE J.; CHICK, LACY D.; KRISHNAN, NIKHIL P.; MCCLURE, ERIN; PELESKO, JULIA; WILLIAMSON, DREW F. K.; NICHOL, DANIEL; MANDAL, SOUMYAJIT; BONOMO, ROBERT A.; SCOTT, JACOB G.
- Abstract
A morbidostat is a bioreactor that uses antibiotics to control the growth of bacteria, making it well-suited for studying the evolution of antibiotic resistance. However, morbidostats are often too expensive to be used in educational settings. Here we present a low-cost morbidostat called the EVolutionary biorEactor (EVE) that can be built by students with minimal engineering and programming experience. We describe how we validated EVE in a real classroom setting by evolving replicate Escherichia coli populations under chloramphenicol challenge, thereby enabling students to learn about bacterial growth and antibiotic resistance.
- Subjects
DRUG resistance in bacteria; CHLORAMPHENICOL; ESCHERICHIA coli; ANTIBIOTICS; ENGINEERING students
- Publication
eLife, 2022, p1
- ISSN
2050-084X
- Publication type
Product Review
- DOI
10.7554/eLife.83067