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- Title
A new RNA polymerase sigma factor,σ<sup>F</sup>, is required for the late stages of morphological differentiation in Streptomyces spp.
- Authors
Potúc̆ková, Laura; Kelemen, Gabriella H.; Findlay, Kim C.; Lonetto, Michael A.; Buttner, Mark J.; Kormanec, Ján
- Abstract
A gene (<em>sigF</em>) encoding a new sigma factor was isolated from <em>Streptomyces aureofaciens</em> using a degenerate oligonucleotide probe designed from the GLI(KDNE)A motif lying within the well-conserved region 2.2 of the eubacterial σ70 family. Homologues were present in other <em>Streptomyces</em> spp., and that of the genetically well-studied <em>Streptomyces coelicolor</em> A3(2) was also cloned. The nucleotide sequences of the two sigF genes were determined and shown to encode primary translation products of 287 (<em>S. coelicolor</em>) and 295 (<em>S. aureofaciens</em>) amino acid residues, both showing greatest similarity to σB of <em>Bacillus subtilis</em>. However, while σB is involved in stationary-phase gene expression and in the general stress response in <em>B. subtilis</em>, σF affects morphological differentiation in <em>Streptomyces</em>. Disruption of <em>sigF</em> did not affect vegetative growth but did cause a <em>whi</em> mutant phenotype. Microscopic examination showed that the <em>sigF</em> mutant produced spores that were smaller and deformed compared with those of the wild type, that the spore walls were thinner and sensitive to detergents and that in <em>sigF</em> mutant spores the chromosome
- Subjects
NUCLEOTIDE sequence; STREPTOMYCES aureofaciens; NUCLEIC acid probes; OLIGONUCLEOTIDES; BACILLUS subtilis
- Publication
Molecular Microbiology, 1995, Vol 17, Issue 1, p37
- ISSN
0950-382X
- Publication type
Article