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- Title
Detection and Characterization of the Metabolites of Ciwujianoside B in Rats Based on UPLC-Fusion Lumos Orbitrap Mass Spectrometry.
- Authors
Dong, Wan-Ru; Gao, Xue; Li, Chen-Xue; Song, Yan; Chai, Jun-Hong; Liang, Jun
- Abstract
We previously conducted a systematic study on the metabolic process and products of hederasaponin B in rats. We hypothesized that the sugar chain structures play a key role in the metabolism of triterpenoid saponins. To verify this hypothesis, we conducted metabolic research on ciwujianoside B ascribed to the same sugar chains and a distinct aglycone and compared it with hederasaponin B. Specifically, we collected feces, urine, and plasma of rats after gavage with ciwujianoside B and identified 42 metabolites by UPLC-Fusion Lumos Orbitrap mass spectrometry. Finally, ciwujianoside B metabolism and hederasaponin B metabolism were compared, reaching the following conclusions: (i) more than 40 metabolites were identified in both, with the majority of metabolites identified in feces; (ii) the corresponding metabolic pathways in vivo were basically similar, including deglycosylation, acetylation, hydroxylation, glucuronidation, oxidation, and glycosylation; and (iii) deglycosylation was considered the main metabolic reaction, and its metabolites accounted for approximately 50% of all metabolites. Overall, this study provides a foundation for further research on the metabolism of triterpenoid saponins.
- Subjects
MASS spectrometry; METABOLITES; TRITERPENOID saponins; QUADRUPOLE ion trap mass spectrometry; RATS; LIQUID chromatography-mass spectrometry; SAPONINS; GLUCURONIDATION; TRITERPENOIDS
- Publication
Journal of Analytical Methods in Chemistry, 2024, Vol 2024, p1
- ISSN
2090-8865
- Publication type
Article
- DOI
10.1155/2024/3187511