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- Title
DNA–Gold Nanozyme-Modified Paper Device for Enhanced Colorimetric Detection of Mercury Ions.
- Authors
Mao, Min-Xin; Zheng, Rong; Peng, Chi-Fang; Wei, Xin-Lin
- Abstract
In this work, a paper device consisted of a patterned paper chip, wicking pads, and a base was fabricated. On the paper chip, DNA–gold nanoparticles (DNA–AuNPs) were deposited and Hg2+ ions could be adsorbed by the DNA–AuNPs. The formed DNA–AuNP/Hg2+ nanozyme could catalyze the tetramethylbenzidine (TMB)–H2O2 chromogenic reaction. Due to the wicking pads, a larger volume of Hg2+ sample could be applied to the paper device for Hg2+ detection and therefore the color response could be enhanced. The paper device achieved a cut-off value of 50 nM by the naked eye for Hg2+ under optimized conditions. Moreover, quantitative measurements could be implemented by using a desktop scanner and extracting grayscale values. A linear range of 50–2000 nM Hg2+ was obtained with a detection limit of 10 nM. In addition, the paper device could be applied in the detection of environmental water samples with high recoveries ranging from 85.7% to 105.6%. The paper-device-based colorimetric detection was low-cost, simple, and demonstrated high potential in real-sample applications.
- Subjects
MERCURY; ENVIRONMENTAL sampling; IONS; WATER sampling; DETECTION limit; METHYLMERCURY
- Publication
Biosensors (2079-6374), 2020, Vol 10, Issue 12, p211
- ISSN
2079-6374
- Publication type
Article
- DOI
10.3390/bios10120211