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- Title
Effect of variation in RANTES promoter on serum RANTES levels and risk of recurrent wheezing after RSV bronchiolitis in children from Han, Southern China.
- Authors
Man Tian; Feng Liu; Guan-yu Wen; Sheng-yun Shi; Rong-hua Chen; De-yu Zhao; Tian, Man; Liu, Feng; Wen, Guan-yu; Shi, Sheng-yun; Chen, Rong-hua; Zhao, De-yu
- Abstract
To investigate the association among RANTES (regulated on activation normal T cell expressed and secreted) gene promoter polymorphism, serum RANTES levels, and recurrent wheezing after RSV (respiratory syncytial virus) bronchiolitis in children (1-12 months of age) from Han, Southern china. Three hundred twenty children with RSV bronchiolitis and 272 controls were enrolled in the 3-year follow-up study. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RELP), enzyme-linked immunosorbent assay (ELISA) kit and luciferase analysis were the mainly used methods, which were used to genotype the RANTES (-403 G/A), assess the serum RANTES levels and the RANTES promoter activity. As the results showed, the RANTES (-403 G/A) in the promoter region was associated with recurrent wheezing after RSV bronchiolitis (p < 0.05) and serum RANTES levels (RANTES genotype G/G: 26.03 +/- 7.46 ng/ml G/A: 28.22 +/- 6.44 ng/ml A/A: 30.12 +/- 5.88 ng/ml). Functional analyses of RANTES promoter activity indicated that the RANTES (-403 G to A) mutation increases the transcriptional activity of the RANTES promoter. In conclusion, the RANTES (-403 G/A) polymorphism increases RANTES transcriptional activity resulted in a high serum RANTES levels, thus increased the risk of recurrent wheezing after RSV bronchiolitis.
- Subjects
CHINA; RESPIRATORY syncytial virus; GENES; WHEEZE; JUVENILE diseases; POLYMERASE chain reaction; GENETIC mutation; BIOLOGICAL variation; ASIANS; ASTHMA; COMPARATIVE studies; CYTOKINES; DISEASE susceptibility; GENETIC polymorphisms; LONGITUDINAL method; RESEARCH methodology; MEDICAL cooperation; RESEARCH; RESPIRATORY organ sounds; DISEASE relapse; EVALUATION research; CASE-control method; RESPIRATORY syncytial virus infections
- Publication
European Journal of Pediatrics, 2009, Vol 168, Issue 8, p963
- ISSN
0340-6199
- Publication type
journal article
- DOI
10.1007/s00431-008-0870-3