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- Title
Empowering the on-site detection of nucleic acids by integrating CRISPR and digital signal processing.
- Authors
Lee, Chang Yeol; Kim, Hyunho; Degani, Ismail; Lee, Hanna; Sandoval, Angel; Nam, Yoonho; Pascavis, Madeleine; Park, Hyun Gyu; Randall, Thomas; Ly, Amy; Castro, Cesar M.; Lee, Hakho
- Abstract
Addressing the global disparity in cancer care necessitates the development of rapid and affordable nucleic acid (NA) testing technologies. This need is particularly critical for cervical cancer, where molecular detection of human papillomavirus (HPV) has emerged as an accurate screening method. However, implementing this transition in low- and middle-income countries has been challenging due to the high costs and centralized facilities required for current NA tests. Here, we present CreDiT (CRISPR Enhanced Digital Testing) for on-site NA detection. The CreDiT platform integrates i) a one-pot CRISPR strategy that simultaneously amplifies both target NAs and analytical signals and ii) a robust fluorescent detection based on digital communication (encoding/decoding) technology. These features enable a rapid assay (<35 minutes) in a single streamlined workflow. We demonstrate the sensitive detection of cell-derived HPV DNA targets down to single copies and accurate identification of HPV types in clinical cervical brushing specimens (n = 121). Developing rapid and affordable HPV tests is imperative for effective cervical cancer screening in resource-limited regions. Here, the authors demonstrate an advanced HPV diagnostic system that integrates CRISPR technology with digital signal processing.
- Subjects
DIGITAL signal processing; CRISPRS; RESOURCE-limited settings; HUMAN papillomavirus; DIGITAL communications; NUCLEIC acids
- Publication
Nature Communications, 2024, Vol 15, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-024-50588-3