We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Mapping recurrent mosaic copy number variation in human neurons.
- Authors
Sun, Chen; Kathuria, Kunal; Emery, Sarah B.; Kim, ByungJun; Burbulis, Ian E.; Shin, Joo Heon; Gleeson, Joseph G.; Breuss, Martin W.; Yang, Xiaoxu; Antaki, Danny; Chung, Changuk; Averbuj, Dan; Ball, Laurel L.; Roy, Subhojit; Weinberger, Daniel; Jaffe, Andrew; Paquola, Apua; Erwin, Jennifer; Straub, Richard; Narurkar, Rujuta
- Abstract
When somatic cells acquire complex karyotypes, they often are removed by the immune system. Mutant somatic cells that evade immune surveillance can lead to cancer. Neurons with complex karyotypes arise during neurotypical brain development, but neurons are almost never the origin of brain cancers. Instead, somatic mutations in neurons can bring about neurodevelopmental disorders, and contribute to the polygenic landscape of neuropsychiatric and neurodegenerative disease. A subset of human neurons harbors idiosyncratic copy number variants (CNVs, "CNV neurons"), but previous analyses of CNV neurons are limited by relatively small sample sizes. Here, we develop an allele-based validation approach, SCOVAL, to corroborate or reject read-depth based CNV calls in single human neurons. We apply this approach to 2,125 frontal cortical neurons from a neurotypical human brain. SCOVAL identifies 226 CNV neurons, which include a subclass of 65 CNV neurons with highly aberrant karyotypes containing whole or substantial losses on multiple chromosomes. Moreover, we find that CNV location appears to be nonrandom. Recurrent regions of neuronal genome rearrangement contain fewer, but longer, genes. Single cell genome sequencing approaches have identified somatic copy number variants (CNVs) in human neurons, but small sample sizes (<100 neurons) have limited the power to find recurrent patterns such as CNV hotspots in a single individual. Here, the authors develop an approach to map CNVs in 2097 neurons from a neurotypical individual, finding that >10% neurons contain at least one somatic CNV, and enabling deeper investigation of these events.
- Subjects
DNA copy number variations; SOMATIC mutation; NEURONS; SOMATIC cells; NEURAL development; FETAL brain
- Publication
Nature Communications, 2024, Vol 15, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-024-48392-0