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- Title
Comparison of phage host range determination techniques for critical priority pathogens indicates spot testing with dilutions is the most resource consumptive: A meta-analysis.
- Authors
Bhogal, Jaden
- Abstract
Antibiotic resistance represents a growing medical crisis and is predicted to cause over 10 million deaths globally per year by 2050. Among the most dangerous resistant bacteria are the critical priority pathogens denoted by the World Health Organization (WHO). Fortunately, phage therapy, a high potential treatment method that uses viruses to infect and lyse bacteria, can successfully clear antibiotic-resistant infections. However, it has not passed clinical trials due to the lack of a streamlined process for phage characterization, highlighting the need to compare phage host range determination (HRD) techniques to simplify this process. To address this, phage primary research papers with HRD data were collected from PubMed and Google Scholar. 56 suitable studies were grouped by critical priority pathogen and employed HRD technique. The quantity (mL) of agar, phage filtrate, and bacterial culture used was recorded. Means for each group were compared using a non-parametric ANOVA. For the E. coli O157:H7 research paper group, plaque testing used significantly less total material and agar than spot testing with dilutions (p 5 0.047, p 5 0.041), while spot testing without dilutions used significantly less phage filtrate than plaque testing (p 5 0.035) and significantly less bacterial culture than spot testing with dilutions (p 5 0.013), strongly suggesting that spot testing with dilutions should not be chosen over other methods when conducting HRD as it is the most resource-consumptive. Further investigation into other phage characterization metrics is warranted.
- Subjects
WORLD Health Organization; ESCHERICHIA coli; DILUTION; BACTERIAL cultures; PATHOGENIC microorganisms; BACTERIOPHAGES; DRUG resistance in bacteria
- Publication
Bios, 2024, Vol 92, Issue 2, p60
- ISSN
0005-3155
- Publication type
Article
- DOI
10.1893/BIOS-D-22-00005