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- Title
SNOM on cell thin sections: observation of Jurkat and MDAMB453 cells.
- Authors
Zweyer, M.; Troian, B.; Spreafico, V.; Prato, S.
- Abstract
In this study a comparison of scanning near-field optical microscopy with a traditional, well-known microscopic technique like transmission electron microscopy is discussed. To establish a reliable and comparable method for high-resolution scanning near-field optical microscopy imaging of biological samples, the attention is focussed on cell sections. In particular, we present a study of ultrathin sections of Jurkat T-cells and MDAMB453 cells. We show the relationship among the scanning near-field optical microscopy (topographic and optical) images and the kind of embedding medium (resin), the sections thickness and the staining of the sample. For a complementary investigation atomic force microscopy measurements are carried out, as well. The study reveals that scanning near-field optical microscopy technique on opportunely prepared thin sections can be applied successfully for investigation of the interior of the cells. Scanning near-field optical microscopy and transmission electron microscopy allow to obtain different, however comparable, and complementary information of the cell sample.
- Subjects
NEAR-field microscopy; SCANNING probe microscopy; T cells; TRANSMISSION electron microscopy; ATOMIC force microscopy
- Publication
Journal of Microscopy, 2008, Vol 229, Issue 3, p440
- ISSN
0022-2720
- Publication type
Article
- DOI
10.1111/j.1365-2818.2008.01925.x