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- Title
Electrochemical immunosensor based on binary nanoparticles decorated rGO-TEPA as magnetic capture and Au@PtNPs as probe for CEA detection.
- Authors
Cao, Liangli; Xiao, Haolin; Fang, Cheng; Zhao, Feijun; Chen, Zhencheng
- Abstract
Using gold and magnetic nanoparticles co-decorated reduced graphene oxide-tetraethylenepentamine (rGO-TEPA/Au-MNPs) as the magnetic platform for capturing the primary antibody (Ab1), separation and preconcentration of immunocomplex, a novel homogeneous electrochemical immunosensor was successfully developed. The newly prepared magnetic rGO-TEPA/Au-MNPs, compared with MNPs, exhibited better stability and enhanced electrical conductivity attributed to rGO-TEPA, and showed higher biorecognition efficiency due to AuNPs. In addition, Au@PtNPs were prepared and modified with secondary antibody (Ab2) as an efficient signal probe for signal readout. Using carcinoembryonic antigen (CEA) as a model analyte, the prepared immunosensor demonstrated satisfactory properties like high stability, good repeatability and selectivity, wide linear range (5.0 pg mL−1~200.0 ng mL−1) as well as low detection limit (1.42 pg mL−1). The homogenous electrochemical immunosensor was applied to the detection of CEA in human serum and was found to exhibit good correlation with the reference method. Thus, the proposed rGO-TEPA/Au-MNPs-based homogenous immunoassay platform might open up a new way for biomarker diagnosis.
- Subjects
MAGNETIC nanoparticles; NANOPARTICLES; GOLD nanoparticles; CARCINOEMBRYONIC antigen; ELECTRIC conductivity; DETECTION limit; CHEMICAL preconcentration; SOLID phase extraction
- Publication
Microchimica Acta, 2020, Vol 187, Issue 10, p1
- ISSN
0026-3672
- Publication type
Article
- DOI
10.1007/s00604-020-04559-2