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- Title
Bioluminescent detection of zearalenone using recombinant peptidomimetic Gaussia luciferase fusion protein.
- Authors
Peltomaa, Riikka; Fikacek, Sabrina; Benito-Peña, Elena; Barderas, Rodrigo; Head, Trajen; Deo, Sapna; Daunert, Sylvia; Moreno-Bondi, María C.
- Abstract
The development of a bioluminescent immunosensor is reported for the determination of zearalenone (ZEA) based on a peptide mimetic identified by phage display. The peptide mimetic GW, with a peptide sequence GWWGPYGEIELL, was used to create recombinant fusion proteins with the bioluminescent Gaussia luciferase (GLuc) that were directly used as tracers for toxin detection in a competitive immunoassay without the need for secondary antibodies or further labeling. The bioluminescent sensor, based on protein G–coupled magnetic beads for antibody immobilization, enabled determination of ZEA with a detection limit of 4.2 ng mL−1 (corresponding to 420 μg kg−1 in food samples) and an IC50 value of 11.0 ng mL−1. The sensor performance was evaluated in spiked maize and wheat samples, with recoveries ranging from 87 to 106% (RSD < 20%, n = 3). Finally, the developed method was applied to the analysis of a naturally contaminated reference matrix material and good agreement with the reported concentrations was obtained. Graphical abstract
- Subjects
CHIMERIC proteins; RECOMBINANT proteins; AMINO acid sequence; DETECTION limit; REFERENCE sources; LUCIFERASES; MYCOTOXINS
- Publication
Microchimica Acta, 2020, Vol 187, Issue 10, p1
- ISSN
0026-3672
- Publication type
Article
- DOI
10.1007/s00604-020-04538-7