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- Title
Sperm chromatin condensation, DNA fragmentation and apoptosis in globozoospermic patients.
- Authors
Ghasemzadeh J.; Talebi A. R.; Khalili M. A.; Halvaei I.; Ashourzadeh S.; Nabi A.
- Abstract
Introduction: Globozoospermia is a severe form of teratozoospermia with very low incidence in infertile patients that is characterized by round sperm head and lack of acrosome. It's considered as one of the important causes of male infertility which the success rate in assisted reproductive technology (ART) cycles is also low. The goal was to compare the semen parameters and chromatin/DNA integrity as well as apoptosis in ejaculated spermatozoa between globozoospermic and normozoospermic men. Materials and Methods: In total 57 men were divided into two groups including globozoospermic (n=27) and normozoospermic men as controls (n=30). Semen analysis was performed according to WHO criteria (2010). Sperm chromatin condensation and DNA integrity were assessed using cytochemical tests including: Aniline blue (AB), Toluidine blue (TB), Chromomycin A3 (CMA3) and Sodium dodecyl sulfate (SDS) for chromatin compaction and Acridine orange (AO), Sperm chromatin dispersion (SCD) and TUNEL assays for DNA structure and apoptosis detection. Results: There were significant differences regarding sperm count, motility and normal morphology between two groups. The percentage of abnormal chromatin packaging/ DNA integrity (using AB, TB, AO and SCD tests) was significantly higher in globozoospermic men compared to normozoospermic samples. The rate of spermatozoa with protamine deficiency (CMA3+) showed an increase in golobozoospermic patients when comparison with controls (63.59±13.29 vs. 24.17±9.5, respectively, pO.OOOl). it should be noted that in SDS test, we didn't any significant difference between groups. But, the rate of TUNEL positive spermatozoa were significantly increased in globozoospermic cases respect to the controls (14.81±9.91 vs. 5.95± 3.02, respectively, p<0.0001). There was no significant correlation between sperm DNA denaturation, DNA fragmentation and apoptosis in globozoospermic men. Our data showed significant correlation between single strand DNA (AO+) and progressive motility (p=0.036). Conclusion: The rate of spermatozoa with abnormal chromatin packaging, DNA damage and apoptosis were significantly higher in globozoospermic samples than normal fertile men. Elowever, the sperm chromatin/ DNA anomalies may be considered as one of the main etiologies of ART failure in these patients.
- Subjects
REPRODUCTIVE technology research; CHROMATIN
- Publication
Iranian Journal of Reproductive Medicine, 2015, p20
- ISSN
1680-6433
- Publication type
Abstract