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- Title
Development of an Organic Dye‐Conjugated β‐Diketonate‐Europium Complex Luminescence Probe for Discrimination and Detection of Intracellular Biothiols.
- Authors
Meng, Xiao; Song, Bo; Zou, Jinhua; Dong, Zhiyuan; Yuan, Jingli
- Abstract
Small molecular biothiols, cysteine (Cys), homocysteine (Hcy) and glutathione (GSH), play important roles in organisms, and their concentration levels are indicative of some human diseases. Herein we report an organic dye‐conjugated β‐diketonate‐Eu3+ complex, [Eu(NBD‐keto)3(DPBT)] (NBD‐keto: 7‐nitro‐2,1,3‐benzoxadiazole (NBD)‐conjugated to 1,1,1,2,2‐pentafluoro‐5‐phenyl‐3,5‐pentanedionate through a "O" ether bond; DPBT: 2‐(N,N‐diethylanilin‐4‐yl)‐4,6‐bis(3,5‐dimethylpyrazol‐1‐yl)‐1,3,5‐triazine), which acts as a unique luminescent probe for detecting and discriminating biothiols. [Eu(NBD‐keto)3(DPBT)] itself is not luminescent due to intramolecular interactions between NBD and β‐diketonate‐Eu3+ moieties. Upon reaction with biothiols, the β‐diketonate‐Eu3+ complex [Eu(keto)3(DPBT)] is generated, which emits long‐lived red emission at 610 nm. Meanwhile, three biothiol‐substituted NBD derivatives that exhibit different luminescence behaviors, green emissive (short‐lived) NBD‐NR (R=Cys or Hcy) at 540 nm and non‐luminescent NBD‐SR (R=GSH), are also generated. These luminescence response behaviors allow time‐gated and steady‐state luminescence modes to be combined for detecting total biothiols and discriminating GSH and Cys/Hcy. Using this probe, the quantitative detection and discrimination of GSH and Cys/Hcy in lysis solutions of HeLa cells were realized, which revealed the potential of the probe for biomedical applications.
- Subjects
CYSTEINE; LUMINESCENCE; LUMINESCENT probes; HELA cells; TRIAZINES; HOMOCYSTEINE; FLUORESCENT probes
- Publication
European Journal of Inorganic Chemistry, 2023, Vol 26, Issue 4, p1
- ISSN
1434-1948
- Publication type
Article
- DOI
10.1002/ejic.202200628