We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
IL23R gene polymorphism with juvenile idiopathic arthritis and its association with serum IL-17A.
- Authors
Emami, Samira; Ziaee, Vahid; Rezaei, Arezou; Sadr, Maryam; Maddah, Marzieh; Amirzargar, Ali Akbar; Rezaei, Nima
- Abstract
Aim Interleukin 23 ( IL-23) and its receptor ( IL-23R) seem to play a major role in differentiation of CD4+ T cells into Th17 cells, induction of IL-17 production, and activation of inflammatory pathways. Recent studies have suggested the association of IL-23R polymorphisms with bone and articular inflammation in diseases such as ankylosing spondylitis and rheumatoid arthritis. The aim of this study was to determine the association between IL-23R polymorphisms and juvenile idiopathic arthritis ( JIA). Method A case-control study on 55 patients with JIA and 78 healthy controls was performed. All samples were genotyped for eight single nucleotide polymorphisms ( SNPs) of IL23R (rs1004819, rs2201841, rs10889677, rs1495965, rs7517847, rs10489629, rs11209026 and rs1343151), using real-time polymerase chain reaction Taqman genotyping technique. Forty-two patients and 42 healthy controls were chosen randomly to measure the level of serum IL-17A using enzyme-linked immunosorbent assay. Results Although the heterozygous genotype of rs1004819 ( GA) showed a weak, but statistically significant protective effect on polyarticular subtype ( P = 0.03), none of the selected SNPs were associated with JIA overall. Indeed the analysis of haplotypes did not show any significant association with JIA. Serum IL-17A level was not significantly different among patients and healthy controls and between JIA subtypes, as well. Moreover, there was no significant correlation between SNPs and serum IL-17A concentration. Conclusion This is the first study of the IL-23R gene in Iranian patients with JIA. Our results did not show any strong association between IL-23R polymorphisms and JIA disease or serum IL-17A levels. The only association was seen between rs1004819 and polyarticular JIA. Further larger studies may help clarify the role, if any, of the IL-23/ IL-17 pathway in the pathogenesis of JIA.
- Subjects
BODY fluids; JOINT diseases; RHEUMATISM; T cells; ANKYLOSING spondylitis
- Publication
International Journal of Rheumatic Diseases, 2016, Vol 19, Issue 11, p1189
- ISSN
1756-1841
- Publication type
Article
- DOI
10.1111/1756-185X.12674