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- Title
Increased prevalence of interleukin-17-producing CD4<sup>+</sup> tumor infiltrating lymphocytes in human oral squamous cell carcinoma.
- Authors
Lee, Jang-Jaer; Chang, Yen-Liang; Lai, Wan-Ling; Ko, Jenq-Yuh; Kuo, Mark Yen-Ping; Chiang, Chun-Pin; Azuma, Miyuki; Chen, Ching-Wen; Chia, Jean-San
- Abstract
Background. T helper 17 (Th17) and regulatory T cells share plasticity in the expression of interleukin (IL)-17 and forkhead box P3 (FOXP3), but their mutual presence in human diseases is unclear. Methods. IL-17 and FOXP3 were analyzed by immunohistostaining and flow cytometry. The cytokine milieu was analyzed by quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Results. Oral squamous cell carcinoma expresses high levels of IL-1β, IL-6, and transforming growth factor (TGF)-β. A unique subset of FOXP3+ IL-17-producing CD4+ T cells was consistently identified in tumor-infiltrating lymphocytes from advanced stages of cancer, but not in the circulation, at a frequency of 0.5% to 5.5 % of total CD4+ T and positively correlated with the frequency of IL-17+FOXP3− T cells. The IL-17+FOXP3+ T cells express CCR6 and suppress the proliferation of autologous CD4+ CD25− responder T-cells in vitro. Conclusions. The prevalence of IL-17-producing FOXP3+ CD4+ tumor infiltrating lymphocytes is increased in oral squamous cell carcinoma. © 2010 Wiley Periodicals, Inc. Head Neck, 2010
- Subjects
INTERLEUKINS; LYMPHOCYTE receptors; SQUAMOUS cell carcinoma; T cell differentiation; DIAGNOSTIC use of flow cytometry; REVERSE transcriptase polymerase chain reaction; CELL proliferation
- Publication
Head & Neck, 2011, Vol 33, Issue 9, p1301
- ISSN
1043-3074
- Publication type
Article
- DOI
10.1002/hed.21607