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- Title
Antitumor activity of satraplatin in cisplatin-resistant oral squamous cell carcinoma cells.
- Authors
Yamano, Yukio; Shiiba, Masashi; Negoro, Kenji; Nakatani, Ken; Kasamatsu, Atsushi; Yamatoji, Masanobu; Sakuma, Kentaro; Ogoshi, Kenji; Iyoda, Manabu; Shinozuka, Keiji; Yokoe, Hidetaka; Wada, Takeshi; Fujita, Shigeyuki; Iwasawa, Shunichiro; Takiguchi, Yuichi; Tanzawa, Hideki; Uzawa, Katsuhiro
- Abstract
Background. The aim of the current study was to identify the antitumor activity of satraplatin in paired cisplatin (CDDP)-resistant oral squamous cell carcinoma (OSCC) cell line and its parental cell line. Methods. CDDP-resistant (KB-R) cells and parental cells (KB) pair were used. Viability was assessed using the MTT and clonogenic assay. Real-time polymerase chain reaction (PCR), glutathione (GSH) assay, and flow cytometric analysis were used for further assessment. Results. KB-R cells did not show cross-resistance to satraplatin. The expression status of almost all transporters was upregulated in the KB-R cells. There was no difference in the GSH levels between the KB and KB-R cells. Flow cytometric analysis indicated that with satraplatin the G2/M phase was arrested in the KB-R cells. KB-R cells contain enriched side population cells. Conclusion. These data suggested that satraplatin has antitumor activity against the CDDP-resistant OSCC cells. The mechanism of cross-resistance to platinum agents seems to be multifactorial.
- Subjects
DRUG resistance in cancer cells; SQUAMOUS cell carcinoma; POLYMERASE chain reaction; CYTOMETRY; CISPLATIN; CELL cycle; CELL lines
- Publication
Head & Neck, 2011, Vol 33, Issue 3, p309
- ISSN
1043-3074
- Publication type
Article
- DOI
10.1002/hed.21445