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- Title
Structural organization of the mitochondrial DNA control region in Aedes aegypti.
- Authors
Dueñas, Juan C. Rondan; Gardenal, Cristina N.; Llinás, Guillermo Albrieu; Panzetta-Dutari, Graciela M.
- Abstract
The complete A+T - rich region of Aedes aegypti mitochondrial DNA has been cloned and sequenced. In Argentinean populations of the species, a polymorphism in the length of the amplified fragment was observed. Nucleotide sequence comparison of the shortest and longest A+T - rich amplified fragments detected revealed the presence of 2 types of tandemly repeated blocks. The size variation observed in natural populations is mainly due to the presence of a variable number of a 181 bp tandem repeat unit, located toward the 12S rRNA gene end. The size of the longest A+T - rich region was of 2070 bp, representing the largest control sequence reported for any mosquito species. Few relevant short blocks of primary-sequence similarity conserved in the control region of mosquitoes and other insects were detected scattered throughout the whole region. Five putative stem-loop secondary structures were found, one of them flanked by conserved sequences described in other insects. Our results suggest that there are no universal models of structure–function relations in the control region of insect mtDNA. In addition, we identified a short A+T - rich variable segment in the Ae. aegyti control region that would be suitable for population genetic studies.
- Subjects
ARGENTINA; MITOCHONDRIAL DNA; AEDES aegypti; GENETIC polymorphisms; MOSQUITOES; POPULATION genetics
- Publication
Genome, 2006, Vol 49, Issue 8, p931
- ISSN
0831-2796
- Publication type
Article
- DOI
10.1139/G06-053