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- Title
Limited Utility of Fluorescence In Situ Hybridization for Recurrent Abnormalities in Acute Myeloid Leukemia at Diagnosis and Follow-up.
- Authors
Wheeler, Ferrin C; Kim, Annette S; Mosse, Claudio A; Shaver, Aaron C; Yenamandra, Ashwini; Seegmiller, Adam C
- Abstract
<bold>Objectives: </bold>Acute myeloid leukemia (AML) is classified in part by recurrent cytogenetic abnormalities, often detected by both fluorescent in situ hybridization (FISH) and karyotype. The goal of this study was to assess the utility of FISH and karyotyping at diagnosis and follow-up.<bold>Methods: </bold>Adult AML samples at diagnosis or follow-up with karyotype and FISH were identified. Concordance was determined, and clinical characteristics and outcomes for discordant results were evaluated.<bold>Results: </bold>Karyotype and FISH results were concordant in 193 (95.0%) of 203 diagnostic samples. In 10 cases, FISH detected an abnormality, but karyotype was normal. Of these, one had a FISH result with clinical significance. In follow-up cases, 17 (8.1%) of 211 showed FISH-positive discordant results; most were consistent with low-level residual disease.<bold>Conclusions: </bold>Clinically significant discordance between karyotype and AML FISH is uncommon. Consequently, FISH testing can safely be omitted from most of these samples. Focused FISH testing is more useful at follow-up, for minimal residual disease detection.
- Subjects
DIAGNOSTIC use of in-situ hybridization; ACUTE myeloid leukemia diagnosis; FOLLOW-up studies (Medicine); CARCINOGENESIS; CHROMOSOME abnormalities; KARYOTYPES; LONGITUDINAL method; FLUORESCENCE in situ hybridization; DISEASE relapse; ACUTE myeloid leukemia
- Publication
American Journal of Clinical Pathology, 2018, Vol 149, Issue 5, p418
- ISSN
0002-9173
- Publication type
journal article
- DOI
10.1093/ajcp/aqy002