We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Impact of p16<sup>INK4a</sup> Immunohistochemistry Staining on Interobserver Agreement on the Diagnosis of Cervical Intraepithelial Neoplasia.
- Authors
Pacchiarotti, Alberto; Galeotti, Simona; Bellardini, Paola; Chini, Francesco; Collina, Guido; Palma, Paolo Dalla; Musolino, Fabio; Maccallini, Vincenzo; Ghiringhello, Bruno; Negri, Giovanni; Pisa, Roberto; Sabatucci, Ilaria; Rossi, Paolo Giorgi
- Abstract
Objectives: This study aimed to compare the interobserver Cohen κ on H&E staining and on H&E plus p16INK4a staining of all cervical biopsy specimens in a population-based screening program. Methods: All the colposcopy-guided biopsies generated by the routine screening of 23,258 women aged 25 to 64 years were stained with H&E and H&E plus p16. Biopsy specimens were reviewed by six external experts. Results: The four diagnoses were available in 441 cases. The interobserver κ values were 0.52 (95% confidence interval [CI], 0.45-0.58) and 0.48 (95% CI, 0.42-0.56) with H&E and H&E + p16, respectively, when using a five-group classification (normal, CIN 1, CIN 2, CIN 3, and cancer); adopting a two-group classification (≤CIN 1 and ≥CIN 2), the values were 0.75 (95% CI, 0.66-0.82) and 0.70 (95% CI, 0.61-0.79), respectively. Conclusions: The use of p16 on all cervical biopsy specimens in a screening program showed virtually no effect on reproducibility of the histologic diagnosis.
- Subjects
CYCLIN-dependent kinase inhibitors; CERVICAL cancer research; MEDICAL screening; IMMUNOHISTOCHEMISTRY; CERVICAL intraepithelial neoplasia; COLPOSCOPY
- Publication
American Journal of Clinical Pathology, 2014, Vol 141, Issue 3, p367
- ISSN
0002-9173
- Publication type
Article
- DOI
10.1309/AJCPCYWVL61SVKFU