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- Title
Model-based Traction Force Microscopy Reveals Differential Tension in Cellular Actin Bundles.
- Authors
Soiné, Jérôme R. D.; Brand, Christoph A.; Stricker, Jonathan; Oakes, Patrick W.; Gardel, Margaret L.; Schwarz, Ulrich S.
- Abstract
Adherent cells use forces at the cell-substrate interface to sense and respond to the physical properties of their environment. These cell forces can be measured with traction force microscopy which inverts the equations of elasticity theory to calculate them from the deformations of soft polymer substrates. We introduce a new type of traction force microscopy that in contrast to traditional methods uses additional image data for cytoskeleton and adhesion structures and a biophysical model to improve the robustness of the inverse procedure and abolishes the need for regularization. We use this method to demonstrate that ventral stress fibers of U2OS-cells are typically under higher mechanical tension than dorsal stress fibers or transverse arcs.
- Subjects
STRESS fibers (Cytology); ACTIN; FOCAL adhesions; CELL-matrix adhesions; MICROSCOPY; CYTOSKELETON; EXTRACELLULAR matrix; OSTEOSARCOMA
- Publication
PLoS Computational Biology, 2015, Vol 11, Issue 3, p1
- ISSN
1553-734X
- Publication type
Article
- DOI
10.1371/journal.pcbi.1004076