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- Title
Proteomic Analysis of Sera from Common Variable Immunodeficiency Patients Undergoing Replacement Intravenous Immunoglobulin Therapy.
- Authors
Spadaro, Giuseppe; D'Orio, Concetta; Genovese, Arturo; Galeotafiore, Antonella; D'Ambrosio, Chiara; Di Giovanni, Stefano; Vitale, Monica; Capasso, Mario; Lamberti, Vincenzo; Scaloni, Andrea; Gianni Marone; Zambrano, Nicola
- Abstract
Common variable immunodeficiency is the most common form of symptomatic primary antibody failure in adults and children. Replacement immunoglobulin is the standard treatment of these patients. By using a differential proteomic approach based on 2D-DIGE, we examined serum samples from normal donors and from matched, naive, and immunoglobulin-treated patients. The results highlighted regulated expression of serum proteins in naive patients. Among the identified proteins, clusterin/ApoJ serum levels were lower in naive patients, compared to normal subjects. This finding was validated in a wider collection of samples from newly enrolled patients. The establishment of a cellular system, based on a human hepatocyte cell line HuH7, allowed to ascertain a potential role in the regulation of CLU gene expression by immunoglobulins.
- Publication
Journal of Biomedicine & Biotechnology, 2011, p1
- ISSN
1110-7243
- Publication type
Article
- DOI
10.1155/2011/706746