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- Title
Early enforcement of cell identity by a functional component of the terminally differentiated state.
- Authors
Bahrami-Nejad, Zahra; Zhang, Zhi-Bo; Tholen, Stefan; Sharma, Sanjeev; Rabiee, Atefeh; Zhao, Michael L.; Kraemer, Fredric B.; Teruel, Mary N.
- Abstract
How progenitor cells can attain a distinct differentiated cell identity is a challenging problem given the fluctuating signaling environment in which cells exist and that critical transcription factors are often not unique to a differentiation process. Here, we test the hypothesis that a unique differentiated cell identity can result from a core component of the differentiated state doubling up as a signaling protein that also drives differentiation. Using live single-cell imaging in the adipocyte differentiation system, we show that progenitor fat cells (preadipocytes) can only commit to terminally differentiate after up-regulating FABP4, a lipid buffer that is highly enriched in mature adipocytes. Upon induction of adipogenesis in mouse preadipocyte cells, we show that after a long delay, cells first abruptly start to engage a positive feedback between CEBPA and PPARG before then engaging, after a second delay, a positive feedback between FABP4 and PPARG. These sequential positive feedbacks both need to engage in order to drive PPARG levels past the threshold for irreversible differentiation. In the last step before commitment, PPARG transcriptionally increases FABP4 expression while fatty acid-loaded FABP4 increases PPARG activity. Together, our study suggests a control principle for robust cell identity whereby a core component of the differentiated state also promotes differentiation from its own progenitor state. How can progenitor cells attain a distinct differentiated cell identity, given the fluctuating signaling environment and redundancy of critical transcription factors? This study identifies a control principle whereby progenitor cells induced to differentiate can reach the desired distinct differentiated cell fate despite the possibility of many alternative fates en route.
- Subjects
PROGENITOR cells; TRANSCRIPTION factors; ADIPOGENESIS; ROBUST control; FAT cells
- Publication
PLoS Biology, 2022, Vol 20, Issue 12, p1
- ISSN
1544-9173
- Publication type
Article
- DOI
10.1371/journal.pbio.3001900