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- Title
Direct Electrochemical Sensing of Phenazine-1-carboxylic Acid Secreted by Pseudomonas chlororaphis subsp. aureofaciens BCRC 11057<sup>T</sup> Using Disposable Screen-printed Carbon Electrode.
- Authors
Hameed, Asif; Pi, Hong‐Wei; Lin, Shih‐Yao; Lai, Wei‐An; Young, Li‐Sen; Liu, You‐Cheng; Shen, Fo‐Ting; Young, Chiu‐Chung
- Abstract
A novel electrochemical approach for direct recognition of antibiotic phenazine-1-carboxylic acid (PCA) was developed. PCA was electropolymerized on preanodized screen-printed carbon electrode (SPCE*-PCA) through repetitive cyclic voltammetry and characterized by XPS and FESEM. Electron transfer involved intermediate phenomenon of diffusion-controlled redox process and surface bound redox reaction. At pH 8 (optimum), SPCE*-PCA had a detection limit of 0.51±0.04 μM, a quantification limit of 1.7±0.13 μM, linearity of up to 50 µM, a repeatability of 15.5 % and a reproducibility of 1.7 %. PCA secreted by Pseudomonas chlororaphis subsp. aureofaciens BCRC 11057T was investigated successfully using present single run approach.
- Subjects
PHENAZINE; CARBOXYLIC acids; CARBON electrodes; ELECTROCHEMICAL research; ELECTROPOLYMERIZATION; PSEUDOMONAS aeruginosa
- Publication
Electroanalysis, 2016, Vol 28, Issue 4, p846
- ISSN
1040-0397
- Publication type
Article
- DOI
10.1002/elan.201500278