We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Separation and purification of quinolyridine alkaloids from seeds of Thermopsis lanceolata R. Br. by conventional and pH‐zone‐refining counter‐current chromatography.
- Authors
Ning, Fansheng; Zhu, Heng; Yan, Huijiao; Liu, Jiguo; Aziz, Shahid; Hussain, Hidayat; Song, Xiangyun; Xie, Lei; Meng, Zhaoqing; Cao, Guiyun; Wang, Daijie
- Abstract
In this work, the preparative separation of quinolyridine alkaloids from seeds of T. lanceolata by conventional and pH‐zone‐refining counter‐current chromatography. Traditional counter‐current chromatography separation was performed by a flow‐rate changing strategy with a solvent system of ethyl acetate‐n‐butanol‐water (1:9:10, v/v) and 200 mg sample loading. Meanwhile, the pH‐zone‐refining mode was adopted for separating 2.0 g crude alkaloid extracts with the chloroform‐methanol‐water (4:3:3, v/v) solvent system using the stationary and mobile phases of 40 mM hydrochloric acid and 10 mM triethylamine. Finally, six compounds, including N‐formylcytisine (two conformers) (1), N‐acetycytisine (two conformers) (2), (‐)‐cytisine (3), 13‐β‐hydroxylthermopsine (4), N‐methylcytisine (5), and thermopsine (6) were successfully obtained in the two counter‐current chromatography modes with the purities over 96.5%. Moreover, we adopted nuclear magnetic resonance and mass spectrometry for structural characterization. Based on the obtained findings, the pH‐zone‐refining mode was the efficient method to separate quinolyridine alkaloids relative to the traditional mode.
- Subjects
COUNTERCURRENT chromatography; NUCLEAR magnetic resonance; ALKALOIDS; ISOQUINOLINE alkaloids; SEEDS; MASS spectrometry; HYDROCHLORIC acid
- Publication
Journal of Separation Science, 2023, Vol 46, Issue 16, p1
- ISSN
1615-9306
- Publication type
Article
- DOI
10.1002/jssc.202300053