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- Title
Parental environmental exposure leads to glycometabolic disturbances that affect fertilization of eggs in the silkworm Bombyx mori: the parental transcript legacy.
- Authors
Tao, H.; Liu, H.; Cheng, Y.; Sima, Y.; Yin, W.; Xu, S.
- Abstract
Parental transcript legacy plays an important role in fertilization and development of the early embryo. Parental environmental exposure affects the fertilization of eggs, but the underlying biochemical mechanism is largely unresolved. In this study, the parental environmental effects on fertilization of eggs were explored in the silkworm Bombyx mori ( B. mori), an ideal lepidopteran animal model. The results showed that the rate of fertilization decreased after the parents were exposed to a poor environment at 32 °C with continuous illumination for 72 h on days 6-9 of the pupal stage, which is a key period for germ cell maturation. This was likely attributable to lower energy charge values, obstructed nicotinamide adenine dinucleotide (NAD) regeneration and inactive tricarboxylic acid cycle (TCA), leading to accumulation of large amounts of pyruvic acid and lactic acid. This effect was related to energy metabolism via glycolysis; in particular disruption of pyruvate metabolism. In conclusion, this study showed parental exposure to an abnormal environment during germ cell maturation affected glycolysis and the subsequent fertilization of eggs via the parental transcript legacy in B. mori.
- Subjects
SILKWORMS; INSECT metabolism; FERTILIZATION (Biology); ENVIRONMENTAL exposure; ENERGY metabolism regulation; TRICARBOXYLIC acids; PHYSIOLOGY
- Publication
Journal of Comparative Physiology B: Biochemical, Systemic & Environmental Physiology, 2015, Vol 185, Issue 1, p47
- ISSN
0174-1578
- Publication type
Article
- DOI
10.1007/s00360-014-0864-6