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- Title
Avoidance of ribonucleotide-induced mutations by RNase H2 and Srs2-Exo1 mechanisms.
- Authors
Potenski, Catherine J.; Niu, Hengyao; Sung, Patrick; Klein, Hannah L.
- Abstract
Srs2 helicase is known to dismantle nucleofilaments of Rad51 recombinase to prevent spurious recombination events and unwind trinucleotide sequences that are prone to hairpin formation. Here we document a new, unexpected genome maintenance role of Srs2 in the suppression of mutations arising from mis-insertion of ribonucleoside monophosphates during DNA replication. In cells lacking RNase H2, Srs2 unwinds DNA from the 5′ side of a nick generated by DNA topoisomerase I at a ribonucleoside monophosphate residue. In addition, Srs2 interacts with and enhances the activity of the nuclease Exo1, to generate a DNA gap in preparation for repair. Srs2-Exo1 thus functions in a new pathway of nick processing-gap filling that mediates tolerance of ribonucleoside monophosphates in the genome. Our results have implications for understanding the basis of Aicardi-Goutières syndrome, which stems from inactivation of the human RNase H2 complex.
- Subjects
RIBONUCLEOTIDES; GENETIC mutation; RIBONUCLEASE H; RAD51 recombinase; NUCLEOTIDE sequence; DNA replication; DNA topoisomerase I
- Publication
Nature, 2014, Vol 511, Issue 7508, p251
- ISSN
0028-0836
- Publication type
Article
- DOI
10.1038/nature13292