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- Title
An enzyme-linked immunosorbent assay and a gold-nanoparticle based immunochromatographic test for amatoxins using recombinant antibody.
- Authors
He, Kuo; Zhang, Xiuyuan; Zhao, Ruiping; Wang, Lixia; Feng, Tingting; Wei, Dong
- Abstract
The authors describe two kinds of rapid assays for the determination of amatoxins in mushrooms. The first is an enzyme-linked immunosorbent assay (ELISA) using horseradish peroxidase. The second is a rapid immunochromatographic assay that uses colloidal gold as a red label (CG-ICA). Both are based on the use of a well-characterized recombinant single chain variable fragment antibody (named scFv-A4). The half-maximum inhibition concentrations (IC50) of α-amanitin, β-amanitin and γ-amanitin are 78, 85 and 90 ng⋅mL‾, and the limits of detection (LODs; for IC15) are 1.9, 2.1 and 2.8 ng⋅mL‾. The method was applied to the determination of amanitins in mushrooms, and the LODs for α-amanitin, β-amanitin and γ-amanitin in mushroom samples were found to be 4.9, 6.4 and 8.3 ng⋅mL‾. The visual minimum detection limits of the optimized CGIA are 4 and 6 ng⋅mL‾ for mushroom samples. The test can be performed within 10 min. The results of the analysis of spiked samples showed that the CG-IA can rapidly and semi-quantitatively quantify amatoxins in mushroom samples on site and at low costs. [Figure not available: see fulltext.]
- Subjects
GOLD nanoparticles; AMATOXINS; RECOMBINANT antibodies; CHROMATOGRAPHIC analysis; ENZYME-linked immunosorbent assay
- Publication
Microchimica Acta, 2016, Vol 183, Issue 7, p2211
- ISSN
0026-3672
- Publication type
Article
- DOI
10.1007/s00604-016-1856-x