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- Title
与灵芝酸生物合成相关的细胞色素 P450 基因的筛选与分析.
- Authors
姜露熙; 王俊杰; 李娜; 徐军伟
- Abstract
Fourteen candidate steroid binding P450 genes involved in the biosynthesis of ganoderic acids(GAs)in Ganoderma lingzhi were first screened by comparison of gene relative expression levels in G. lingzhi between liquid static and shaking cultures using the quantitative fluorescence PCR method, then further screened by correlation analysis between expression level and GA-T content under different culture conditions including shaking, static, static with the addition of calcium and static with nitrogen-limiting, and finally confirmed for activity through the antisense RNA technology. The results showed that expression levels of P450 gene cyp512a3, cyp512a11, cyp5144n1, cyp512u4 and cyp512v2 under the static culture condition were 7.2, 3.7, 3.4, 89.8 and 63.4 folds, respectively, compared to those under the shaking culture condition. Among the five P450 genes, cyp512a3 and cyp512v2 showed a positive correlation between gene expression and GA-T content. Compared to the wild G. lingzhi, the GA-T content in three cyp512v2-silence G. lingzhi strains decreased by 13%, 69% and 66%, respectively. Our results suggested that P450 cyp512v2 may play an important role in the biosynthesis of ganoderic acid T in G. lingzhi.
- Publication
Acta Edulis Fungi, 2018, Vol 25, Issue 2, p35
- ISSN
1005-9873
- Publication type
Article
- DOI
10.16488/j.cnki.1005-9873.2018.02.004