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- Title
pSIR-bsr, a self-inactivating retrovirus vector expressing the blasticidin S-resistance gene.
- Authors
Fujii, Hodaka
- Abstract
Self-inactivating retrovirus vectors are useful tools for generating stable cell lines harbouring designed exogenous sequences but lacking the constitutive transcriptional activity of the long terminal repeats that are usually retained by non-self-inactivating retrovirus vectors. Thus, self-inactivating retrovirus vectors are ideal vehicles for integrated transgenes comprising transcriptional regulatory sequences, and for the genes expressed by these regulatory sequences. This article describes the development of a self-inactivating retrovirus vector retaining a blasticidin S-resistance (bsr) gene. The vector, named pSIR-bsr, would be useful for transducing multiple expression vectors with different selection markers.
- Subjects
RETROVIRUSES; CELL lines; NUCLEOTIDE sequence; IMMUNOPRECIPITATION; ANTIGENS
- Publication
Biology Methods & Protocols, 2021, Vol 6, Issue 1, p1
- ISSN
2396-8923
- Publication type
Article
- DOI
10.1093/biomethods/bpab022