The gnaphosid spider species Leptopilos manolisi (Chatzaki, 2002) is reported for the first time from Türkiye. Hitherto, there is no other known species of the genus Leptopilos Levy, 2009 in the fauna of Türkiye. The new record in this study constitutes the first species of Leptopilos known from Türkiye. Morphological and genital organs of the species were photographed. The locality record where the species was collected is indicated.