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- Title
Sunflower Protein Enzymatic Hydrolysates as a Medium for Vitamin B2 and B12 Biosynthesis.
- Authors
Baurin, Dmitry V.; Epishkina, Julia M.; Baurina, Alexandra V.; Shakir, Irina V.; Panfilov, Victor I.
- Abstract
Lowering the costs of industrial fermentation processes is a current challenge of modern biotechnology. In this sense, has been studied the possibility to replace the conventional medium for vitamin B2 and B12 biosynthesis with new effective medium from renewable plant-based sources. Riboflavin (vitamin B2) and cobalamin (vitamin B12) are essential compounds in humans diets and their demand is increasing globally. Current manufacturing process of these vitamins is unsustainable because of using costly unrenewable resources and generating hazardous wastes. Meat peptone is conventionally used as a nitrogen source during the fermentation process, however it is relatively expensive. Sunflower protein is a secondary product, which can be obtained from sunflower meal after oil extraction, and rich on its nutritional value. The objective of this study was to select and evaluate fermentative potential of sunflower protein and its enzymatic hydrolysates as a nitrogen source for riboflavin and cobalamin biosynthesis by evaluating the kinetic parameters of strains growth, specific substrate consumption and formation of metabolites. The fermentation of strains was carried out using conventional mineral medium with meat peptone in 250 mL Erlenmeyer flasks, and the results obtained were compared with fermentation where sunflower protein and it's enzymatic hydrolysates were used as a nitrogen source instead of meat peptone. It was observed that when compared with meat peptone, some strains show positive effect in growth when nitrogen source was substituted with sunflower protein enzymatic hydrolysates. This allows affirming that sunflower protein and its enzymatic hydrolysates are promising in the use as a nitrogen source for riboflavin and cobalamin biosynthesis. The characteristics and parameters of studied fermentation processes reveals their potential for residue products applications for obtaining products with high added value.
- Subjects
VITAMIN B2; FERMENTATION products industry; VITAMIN B12; METABOLITES; SUNFLOWERS
- Publication
CET Journal - Chemical Engineering Transactions, 2020, Vol 79, p145
- ISSN
1974-9791
- Publication type
Article
- DOI
10.3303/CET2079025