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- Title
Th17 和 Treg 细胞来源的儿茶酚胺在胶原诱导性关节炎中的作用.
- Authors
王小琴; 彭聿平; 刘展; 邱一华
- Abstract
Objective To explore the role of Th17- and Treg-derived catecholamines during collagen-induced arthritis (CIA) progression. Methods Eighteen male DBA/1 mice were randomly divided into control group, CIA model group Ⅰ(day 35) and CIA model group Ⅱ(day 55). The CIA models were induced by type Ⅱ collagen (CⅡ) injection from tails. mRNA expression of Th17 specific transcription factor include ROR-γt, cytokines, IL-17 IL-22, Treg specific transcription factor, Foxp3, cytokines, TGF-β and tyrosine hydroxylase (TH) in lymph nodes were examined by real-time PCR. Co-localization of ROR-γt or Foxp3, with TH, vesicular monoamine transporter-2 (VMAT-2) or monoamine oxidase (MAO) in lymph nodes were observed by immunofluorescence staining. Results In lymph nodes of mice in CIA Ⅰgroup and CIA Ⅱ group, mRNA expression of ROR-γt, IL-17, TH and IL-22 were upregulated, while mRNA expression of Foxp3 and TGF-β expression was downregulated compared to those expression in control group. The upregulated expression of IL-17 was significantly reduced in CIA Ⅱ group compared with that in CIA Ⅰ group. In the lymph nodes of both intact and CIA mice, co-localization of ROR-γt or Foxp3 with TH, VMAT-2 or MAO was seen in some cells. The numbers of cells that are double-positive of ROR-γt/TH,ROR-γt/VMAT-2 and ROR-γt/MAO IL-17 were increased in CIA groups compared to those in con⁃ trol group. And they are significantly reduced in CIA Ⅱgroup compared with those in CIA Ⅰ group. Conclusion The ability to synthesize catecholamines in Th17 cells was increased in lymph node in mice from CIA groups compared to that in control group. The increased catecholamines production from Th17 cells in lymph nodes may be involved in the anti-inflammatory progression in CIA.
- Publication
Tianjin Medical Journal, 2014, Vol 42, Issue 12, p1172
- ISSN
0253-9896
- Publication type
Article
- DOI
10.3969/j.issn.0253-9896.2014.12.006