We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Screening of Proliferation-Related Genes and Pathological Changes in Thiram-Induced Tibial Dyschondroplasia.
- Authors
Jahejo, Ali Raza; Zhang, Chen-Liang; Nabi, Fazul; Kalhoro, Nazeer Hussain; Shah, Qurban Ali; Bhutto, Zohaib Ahmed; Zhao, Jin-Feng; Yu, Jin; Ning, Guan-Bao; Zhang, Ding; Chen, Shu-Ming; Tian, Wen-Xia
- Abstract
Aim and Background. Tibial dyschondroplasia (TD) is a tibiotarsal bone disorder of rapid growing poultry species. The complete pathogenicity of TD remains unknown; therefore, a study was conducted to screen possible role of proliferation-related gene expression in thiram-induced tibial TD chickens. Materials and Methods. Three hundred sixty (n = 360) broiler chickens were equally divided into control (C) and thiram (T) groups. Furthermore, the C and T groups were dividedinto 8-, 9-, 11-, and 13-day-old chickens. Results. Clinically, it was observed that broiler chickens of group T had abnormal posture, gait, and lameness, and histopathological results revealed dead and abnormal chondrocytes of T group on day 6. Real-time qPCR results showed that HDAC1, MTA1, H4, and PCNA genes were significantly expressed (P < 0.05). HDAC1 was upregulated on days 1, 2, 4, and 6 (P < 0.01); MTA1 was upregulated on days 1 and 2 (P < 0.01); H4 was upregulated on days 2 and 4 (P < 0.01), and PCNA was downregulated on days 1, 2, and 4 (P < 0.01). Furthermore, IHC results of HDAC1 protein were significantly (P < 0.01) expressed in proliferative zone of day 1 and hypertrophic zone of day 6. MTA1 protein was significantly (P < 0.01) expressed on days 1, 2, and 6 in all zones, except prehypertrophic zone of day 2. Conclusion. In conclusion, the mRNA expressions of HDAC1, MTA1, H4, and PCNA were differentially expressed in the chondrocytes of thiram-induced TD chickens. HDAC1 and MTA1 protein expression found involved and responsible in the abnormal chondrocytes' proliferation of broiler chicken.
- Subjects
BIRDS; BIOLOGICAL models; POULTRY; ANIMAL experimentation; OSTEOCHONDRODYSPLASIAS; GENES; DESCRIPTIVE statistics; TIBIA; ACYCLIC acids
- Publication
BioMed Research International, 2022, p1
- ISSN
2314-6133
- Publication type
Article
- DOI
10.1155/2022/6209047