We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Thymol and carvacrol determination in a swine feed organic matrix using Headspace SPME-GC-MS.
- Authors
Jonathan Lona-Ramírez, Fernando; Lizeth Hernández-López, Nancy; González-Alatorre, Guillermo; del Carmen Flores-Flores, Teresa; Patiño-Herrera, Rosalba; Francisco Louvier-Hernández, José
- Abstract
In recent years, oregano essential oil has been used as an animal food additive due to its antifungal and antibacterial properties as well as s synthetic antibiotic substitute. It is desirable to develop fast and effective thymol and carvacrol quantification method in a swine feed organic matrix. In this work, a performance comparison between the Soxhlet solvent extraction technique using petroleum ether and ethyl acetate and the head space-solid phase microextraction (HS-SPME) technique is made. A 24 design of experiments is performed for defining HS-SPME parameters: equilibrium temperature of 40 °C, extraction temperature of 40 °C, ionic strength of 0.57 M, and extraction time of 40 min. The HS-SPME method is more efficient for extracting thymol and carvacrol extraction from an organic matrix. Limits of detection and quantification values using Soxhlet extraction with ethyl acetate were 3.7 and 12.5 pL'1 for thymol and 1.4 and 4.7 μg L-1 for carvacrol, respectively; while LOD and LOQ for HS-SPME were 0.9 and 3.1 μg L-1 for thymol and 0.6 and 1.9 μg L-1 for carvacrol, respectively. The head space-solid phase microextraction method has the potential for quality control in the industry for active compounds present in oregano's essential oil as an additive into an organic matrix.
- Subjects
SWINE nutrition; THYMOL; FOOD additives; IONIC strength; ESSENTIAL oils; EXTRACTION techniques; CARVACROL
- Publication
Revista Mexicana de Ciencias Pecuarias, 2023, Vol 14, Issue 1, p78
- ISSN
2007-1124
- Publication type
Article
- DOI
10.22319/rmcp.v14i1.5394