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- Title
Mutation of DEFECTIVE EMBRYO SAC1 results in a low seed-setting rate in rice by regulating embryo sac development.
- Authors
Hu, Xia; Yu, Ping; Zhang, Yingxin; Gao, Zhiqiang; Sun, Bin; Wu, Weixun; Deng, Chenwei; Abbas, Adil; Hong, Yongbo; Sun, Lianping; Liu, Qunen; Xue, Pao; Wang, Beifang; Zhan, Xiaodeng; Cao, Liyong; Cheng, Shihua
- Abstract
The seed-setting rate has a significant effect on grain yield in rice (Oryza sativa L.). Embryo sac development is essential for seed setting; however, the molecular mechanism underlying this process remains unclear. Here, we isolated defective embryo sac1 (des1), a rice mutant with a low seed-setting rate. Cytological examination showed degenerated embryo sacs and reduced fertilization capacity in des1. Map-based cloning revealed a nonsense mutation in OsDES1 , a gene that encodes a putative nuclear envelope membrane protein (NEMP)-domain-containing protein that is preferentially expressed in pistils. The OsDES1 mutation disrupts the normal formation of functional megaspores, which ultimately results in a degenerated embryo sac in des1. Reciprocal crosses showed that fertilization is abnormal and that the female reproductive organ is defective in des1. OsDES1 interacts with LONELY GUY (LOG), a cytokinin-activating enzyme that acts in the final step of cytokinin synthesis; mutation of LOG led to defective female reproductive organ development. These results demonstrate that OsDES1 functions in determining the rice seed-setting rate by regulating embryo sac development and fertilization. Our study sheds light on the function of NEMP-type proteins in rice reproductive development.
- Subjects
FEMALE reproductive organs; MORPHOGENESIS; EMBRYOS; NONSENSE mutation; NUCLEAR membranes; SEED yield; SEED production (Botany); RICE
- Publication
Journal of Experimental Botany, 2023, Vol 74, Issue 5, p1501
- ISSN
0022-0957
- Publication type
Article
- DOI
10.1093/jxb/erac506