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- Title
DIFFERENTIATION OF CELLULAR AND BIOCHEMICAL FEATURES OF THE SINGLE-CELL C<sub>4</sub> SYNDROME DURING LEAF DEVELOPMENT IN BIENERTIA CYCLOPTERA (CHENOPODIACEAE).
- Authors
Voznesenskaya, Elena V.; Koteyeva, Nouria K.; Chuong, Simon D. X.; Akhani, Hossein; Edwards, Gerald E.; Franceschi, Vincent R.
- Abstract
The terrestrial plant Bienertia cycloptera has been shown to accomplish C4 photosynthesis within individual chlorenchyma cells by spatially separating the phases of carbon assimilation into distinct peripheral and central compartments. in this study, anatomical, physiological, and biochemical techniques were used to determine how this unique compartmentation develops. Western blots show ribulose-1,5-bisphosphate carboxylase (Rubisco) (chloroplastic) is present in the youngest leaves and increases during development, while levels of C4 enzymes—pyruvate,Pi dikinase (chloroplastic), phosphoenolpyruvate carboxylase (PEPC) (cytosol), and NAD-malic enzyme (mitochondrial)—increase later in development. Immunolocalization confirmed this for Rubisco and PEPC. The youngest chlorenchyma cells have a central nucleus surrounded by monomorphic granal chloroplasts containing Rubisco. Later stages show progressive development of a central cytoplasmic compartment enriched with chloroplasts and mitochondria and of a peripheral cytoplasm with chloroplasts. A complex reticulum of connections between the compartments also developed and was characterized. δ13C isotope analyses show mature leaves have distinct C4-type isotope composition, while the composition in younger leaves is "C4-like." Based on the results, this form of single-cell C4 photosynthesis develops from a common pool of organelles through partitioning to separate compartments, and the development of biochemically and ultrastructurally dimorphic chloroplasts.
- Subjects
CYCLOPTERUS; PHOTOSYNTHESIS; PLANT cell culture; LEAF development; PLANT development
- Publication
American Journal of Botany, 2005, Vol 92, Issue 11, p1784
- ISSN
0002-9122
- Publication type
Article
- DOI
10.3732/ajb.92.11.1784