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- Title
In Vitro Antioxidant and Anti-Mycobacterial Activities of Roselle (Hibiscus sabdariffa L.) Calyx Extract against Clinical Isolate of Multidrug Resistant Mycobacterium tuberculosis.
- Authors
Sartini, Sartini; Djide, M. Natsir; Alam, Gemini; Arjuna, Andi; Djide, Nana Juniarti N.
- Abstract
Hibiscus sabdariffa L. or roselle calyx has been known to have biological activities including antioxidant and antibacterial. In this research, we examine the antioxidant activity using 1,1-diphenyl-2- picrylhydrazyl (DPPH) as scavenging agent and the anti-mycobacterial activity towards clinical isolate of multidrug-resistant Mycobacterium tuberculosis (MDR-TB). Roselle calyces were dried and extracted through infusion methods using distilled water, and maceration methods using 50% ethanol, within the ratio of sample (1): solvent (10), followed by spray-drying using maltodextrin as diluting agent. The total phenolic content (TPC) was analyzed using Folin-Ciocalteu method calculated as gallic acid equivalent (GAE), antioxidant activity was analyzed using DPPH method, and anti-mycobacterial activity was assay using Microscopic Observation Drug Susceptibility (MODS) method. As the results, TPC of infusion extract and ethanolic extract were 17.70+0.7 mg GAE/g and 13.15+0.3 mg GAE/g, respectively and IC50 toward DPPH were 0.88 mg/ml for infusion extract and 3.78 mg/ml for ethanolic extract, respectively. Anti-mycobacterial activity of both extracts expressed as Minimum Inhibitory Concentration (MIC) were 10 mg/ml toward clinical isolate of MDR-TB.
- Publication
Systematic Reviews in Pharmacy, 2020, Vol 11, Issue 5, p36
- ISSN
0975-8453
- Publication type
Article
- DOI
10.31838/srp.2020.5.07