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- Title
Detection of Tn916 Conferring Tetracycline Resistance in Clinical Isolates of Streptococcus pyogenes.
- Authors
Jasim, Hameed M.; Farhan, Abbas Aboud; Hassooni, Hanan Raheem; Hassan, Adil; Alhusseiny
- Abstract
Background: During the past twenty years, tetracycline resistance average has increased in Streptococcus pyogenes in many countries. Pneumococcal resistance to erythromycin and tetracycline is associated with the insertion of the erm(B) into the transposons of the Tn916 family. To get datum that may be beneficial in resolving the diffusion of antimicrobial resistance, therefore, we can be specified antibiotic resistant genes and their corporation with mobile genetic elements. This study was proceed to explore the genetic regulation of Tn916-carrying tet(M) in clinical isolates of S.pyogenes . Methods: A twenty two of S.pyogenes isolates were assemble from patients suffering from upper respiratory infection, and the susceptibility of these isolates to tetracycline antibiotics was examined. Molecular detection of Tn916 was carried out by employ certain primers to amplify tet(M) gene in each isolates. Results: The results appeared that the resistance of the tetracycline group was 68.1%, 54.5%, 36.3% and 31.8% for minocycline, tetracycline, oxytetracycline and doxycycline, respectively. Genetic analysis showed that Tn916 was detected in eight of the S.pyogenes clinical isolates resistant to tetracycline. Conclusions: Our findings suggest that clinical isolates of S. pyogenes harboring a copy(s) of Tn916 conferring tetracycline resistance. One possible explanation for resistance to tetracycline in these isolates is due to tet gene, which was most likely located on Tn916.
- Subjects
TETRACYCLINES; STREPTOCOCCUS pyogenes; TETRACYCLINE; MOBILE genetic elements; RESPIRATORY infections; GENETIC regulation; OXYTETRACYCLINE
- Publication
Indian Journal of Forensic Medicine & Toxicology, 2021, Vol 15, Issue 1, p2109
- ISSN
0973-9122
- Publication type
Article
- DOI
10.37506/ijfmt.v15i1.13717