We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Expression of Natural Cytotoxicity Receptors on and Intracellular Cytokine Production by NK Cells in Women with Gestational Diabetes Mellitus.
- Authors
Chiba, Hitomi; Fukui, Atsushi; Fuchinoue, Kohei; Funamizu, Ayano; Tanaka, Kanji; Mizunuma, Hideki
- Abstract
Problem To determine the role of peripheral blood NK ( pNK) cells in putative etiology of gestational diabetes, the expression of surface markers on p NK cells and the percentage of cytokine-producing pNK cells in women at 12 weeks of pregnancy with gestational diabetes mellitus ( GDM) were studied. Method of study Multicolor flow cytometry was used to analyze the expression of NK cell surface receptors ( CD16, NKp46, and NKp30) and intracellular cytokines ( IFN-γ, TNF-α, TGF-β, and VEGF) in pNK cells ( CD56dim and CD56bright) at 12 weeks of pregnancy with GDM ( n = 7) and non- GDM ( n = 28). Results CD56bright/ CD16− NK and CD56bright/ NKp46+ NK cell percentage were significantly lower in GDM women than that in non- GDM women. IFN-γ- and TNF-α-producing CD56+ cells, respectively, were significantly high, while TGF-β- and VEGF-producing CD56+ cells and CD56bright cells, respectively, were significantly low in GDM women. Conclusions Women with GDM possibly have abnormal NK cell function for the expression of surface receptors and cytokine production.
- Subjects
GESTATIONAL diabetes; ANTIBODY-dependent cell cytotoxicity; TRANSFORMING growth factors; KILLER cells; VASCULAR endothelial growth factors; THERAPEUTICS
- Publication
American Journal of Reproductive Immunology, 2016, Vol 75, Issue 5, p529
- ISSN
1046-7408
- Publication type
Article
- DOI
10.1111/aji.12491